Agrobacterium Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Agrobacterium Medium
Composition per liter:
Agar............................................................................................18.0g
Erythritol.......................................................................................5.0g
NaNO 3 ..........................................................................................2.5g
CaCl 2 .............................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.2g
KH 2 PO 4 .........................................................................................0.1g
Ferric EDTA ..............................................................................1.3mg
Biotin.............................................................................................2μg
Supplement..............................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Supplement:
Composition per liter:
Cycloheximide............................................................................0.25g
Bacitracin......................................................................................0.1g
Na 2 SeO 3 ........................................................................................0.1g
Tyrothricin .................................................................................1.0mg
Preparation of Supplement: Add components to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except supplement, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Adjust pH to 7.0 with 1N NaOH. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile supplement. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Agrobacterium spe-
cies biotype 2.