MH Medium, 10% (LMG Medium 270)

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

MH Medium, 10%
(LMG Medium 270)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................81.0g
Agar............................................................................................15.0g
Yeast extract................................................................................10.0g
MgSO 4 ·7H 2 O................................................................................9.6g
MgCl 2 ·6H 2 O .................................................................................7.0g
Proteose peptone No.3..................................................................5.0g
KCl................................................................................................2.0g
Glucose.........................................................................................1.0g
CaCl 2 ...........................................................................................0.54g
NaBr.........................................................................................26.0mg
NaHCO 3  solution.....................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ......................................................................................0.06g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to 10.0mL of
distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except NaHCO 3  solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°C. Aseptically add 10.0mL sterile NaHCO 3  solution. Pour into ster-
ile Petri dishes or aseptically distribute into sterile tubes.
Use: For cultivation and maintenance of Chromohalobacter israelensis
and Chromohalobacter canadensis.