MH Medium, 2%

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

MH Medium, 2%

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
NaCl..............................................................................................0.5g
MgSO 4 ·7H 2 O............................................................................0.025g
FeSO 4 ·7H 2 O...............................................................................2.0mg
Glucose solution ......................................................................20.0mL
Methanol, filter sterilized.........................................................20.0mL
pH 7.0–7.5 at 25°C
Glucose Solution:
Composition per 20.0mL:
Glucose.........................................................................................1.5g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except glucose solu-
tion and methanol, to distilled/deionized water and bring volume to
960.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 20.0mL of sterile glucose solution and 20.0mL
of filter-sterilized methanol. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use:  For the cultivation of Methylobacillus fructoseoxidans and Meth-
ylophilus glucoseoxidans.