MH Medium, 10%

[所属分类:培养基配方] [发布时间:2021-5-28] [发布人:网站管理员2] [阅读次数:] [返回]

MH Medium, 10%

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................81.0g
Yeast extract................................................................................10.0g
MgSO 4 ·7H 2 O................................................................................9.6g
MgCl 2 ·6H 2 O .................................................................................7.0g
Proteose peptone No. 3.................................................................5.0g
KCl................................................................................................2.0g
Glucose.........................................................................................1.0g
CaCl 2 ...........................................................................................0.36g
NaBr..........................................................................................0.026g
NaHCO 3  solution .....................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ......................................................................................0.06g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical-
ly add 10.0mL of sterile NaHCO 3 solution. Mix thoroughly. Aseptical-
ly distribute into sterile tubes or flasks.
Use: For the cultivation of Zygomonas mobilis, Salinicoccus hispani-
cus, Salinicoccus roseus, and Pseudomonas beijerinckii.