Methylococcus Medium

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..............................................................................................8.0g
NaNO 3 (20% solution).............................................................10.0mL
L - F salts solution......................................................................10.0mL
Sodium-potassium phosphate
buffer ...................................................................................6.5mL
pH 7.1 ± 0.2 at 25°C
Sodium-Potassium Phosphate Buffer:
Composition per liter:
KH 2 PO 4 .....................................................................................136.0g
NaOH..........................................................................................28.8g
Preparation of Sodium-Potassium Phosphate Buffer: Add
components to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH to 7.1.
L-F Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O (10% solution) ................................................200.0mL
CaCl 2 ·2H 2 O (10% solution).....................................................20.0mL
FeSO 4 (10% solution)..............................................................10.0mL
ZnSO 4 ·7H 2 O (1% solution) .......................................................4.9mL
H 3 BO 3 (1% solution) .................................................................0.6mL
MnSO 4 ·H 2 O (1% solution) ......................................................0.27mL
CuSO 4 ·5H 2 O (1% solution).......................................................0.2mL
Preparation of L-F Salts Solution: Filter sterilize FeSO 4 solution
immediately prior to use. Add all components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1. Au-
toclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes or leave in tubes.
Use:  For the cultivation and maintenance of Methylococcus species.