Methylohalomonas Medium (DSMZ Medium 1171)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylohalomonas Medium
(DSMZ Medium 1171)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .........................................................................................120.0g
K 2 HPO 4 .........................................................................................2.0g
NH 4 Cl ..........................................................................................0.5g
Magnesium sulfate solution.....................................................10.0mL
Thiosulfate solution .................................................................10.0mL
Bicarbonate solution................................................................10.0mL
Vitamin solution.......................................................................10.0mL
Methanol ...................................................................................2.0mL
Trace elements solution SL-6 ...................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO 4 · 7H 2 O................................................................................2.0g
Preparation of Magnesium Sulfate Solution:  Add MgSO 4 · 7H 2 O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Vitamin Solution:
Composition per 10.0mL:
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add vitamin B 12 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Methanol:
Composition per 10.0mL:
Methanol .................................................................................10.0mL
Preparation of Methanol: Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature.
Bicarbonate Solution :
Composition per 10.0mL:
NaHCO 3 ...................................................................................... 11.0g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Thiosulfate Solution:
Composition per 10.0mL:
NaS 2 O 3 ·5H 2 O .............................................................................18.0g
Preparation of Thiosulfate Solution:  Add NaS 2 O 3 ·5H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-
ature.
Preparation of Medium: Add components, except methanol, vita-
min, bicarbonate, thiosulfate, and magnesium sulfate solutions, to dis-
tilled/deionized water and bring volume to 960.0mL. Mix thoroughly.
Distribute into closed vessels with a medium to headspace ratio of 1:5
to 1:10. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature. Aseptically add the methanol, vitamin, bicarbonate, thio-
sulfate, and magnesium sulfate solutions.
Use: For the cultivation of Methylohalomonas spp.