Methylocella silverstris Medium (DSMZ Medium 1181)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylocella silverstris Medium
(DSMZ Medium 1181)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·12H 2 O.......................................................................0.717g
KH 2 PO 4 ·2H 2 O ..........................................................................0.272g
MgSO 4 ·7H 2 O................................................................................0.2g
NaNO 3 .........................................................................................0.2g
CaCl 2 ·2H 2 O ...............................................................................6.0mg
Fe(III)NH 4 -EDTA .....................................................................4.0mg
Phosphate buffer solution..........................................................5.0mL
Trace elements solution.............................................................0.5mL
pH 5.8 ± 0.2 at 25°C
Phosphate Buffer Solution:
Composition per liter:
NaH 2 PO 4 ·2H 2 O.........................................................................28.71g
Na 2 HPO 4 ·12H 2 O.........................................................................5.73g
Preparation of Phosphate Buffer Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Filter sterilize
Trace Elements Solution:
Composition per liter:
Composition per liter:
H 3 BO 3 ......................................................................................30.0mg
CaCl 2 ·2H 2 O .............................................................................20.0mg
ZnSO 4 ·2H 2 O............................................................................10.0mg
MnCl 2 · 4H 2 O...............................................................................3.0mg
Na 2 MoO 4 ·2H 2 O .........................................................................3.0mg
CuCl 2 ·6H 2 O ...............................................................................1.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except phosphate buf-
fer solution, to distilled/deionized water and bring volume to 995.0mL.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature. Aseptically add 5.0mL sterile
phosphate buffer solution. Adjust pH to 5.8.
Use: For the cultivation of Methylocella silverstris.