Methylobacterium Agar

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylobacterium Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
KNO 3 ............................................................................................1.0g
Na 2 HPO 4 .....................................................................................0.23g
MgSO 4 ·7H 2 O............................................................................... 0.2g
NaH 2 PO 4 .....................................................................................0.07g
CaCl 2 ·2H 2 O.................................................................................0.02g
FeSO 4 ·7H 2 O.............................................................................. 1.0mg
ZnSO 4 ·7H 2 O............................................................................ 70.0μg
H 3 BO 3 .......................................................................................10.0μg
MnSO 4 ·5H 2 O............................................................................10.0μg
MoO 3 .........................................................................................10.0μg
CuSO 4 ·5H 2 O.............................................................................. 5.0μg
Methanol, filter sterilized...........................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 995.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 5.0mL of filter-
sterilized methanol. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation and maintenance of Methylobacterium
organophilum.