Methylobacterium Medium (DSMZ Medium 125)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylobacterium Medium
(DSMZ Medium 125)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
KNO 3 ............................................................................................1.0g
Na 2 HPO 4 .....................................................................................0.23g
MgSO 4 ·7H 2 O................................................................................0.2g
NaH 2 PO 4 .....................................................................................0.07g
CaCl 2 ·2H 2 O.................................................................................0.02g
FeSO 4 ·7H 2 O...............................................................................1.0mg
ZnSO 4 ·7H 2 O.............................................................................70.0μg
MoO 3 .........................................................................................10.0μg
H 3 BO 3 .......................................................................................10.0μg
MnSO 4 ·5H 2 O............................................................................10.0μg
CuSO 4 ·5H 2 O...............................................................................5.0μg
Methanol..................................................................................15.0mL
pH 4.0–5.4 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Acidomonas methanolica
and Methanomonas methylovora.