Aeropyrum JXT Medium (DSMZ Medium 820)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aeropyrum JXT Medium
(DSMZ Medium 820)
Composition per liter:
Yeast extract..................................................................................1.0g
Trypticase™ peptone....................................................................1.0g
Na 2 S 2 O 3 ·5H 2 O..............................................................................1.0g
Seawater...............................................................................1000.0mL
pH 7.1 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl........................................................................................23.477g
MgCl 2 ·6H 2 O .............................................................................4.981g
Na 2 SO 4 ......................................................................................3.917g
CaCl 2 ..........................................................................................1.12g
KCl.........................................................................................664.0mg
NaHCO 3 .................................................................................192.0mg
H 3 BO 3 ......................................................................................26.0mg
SrCl 2 ........................................................................................24.0mg
KBr ............................................................................................6.0mg
NaF ............................................................................................3.0mg
Preparation of Artificial Seawater:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to 1.0L artificial seawa-
ter (filtered natural seawater can be used instead of artificial seawater).
Mix thoroughly. Adjust pH to 7.0–7.2. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Aeropyrum pernix.