Aeromonas Medium (Ryan’s Aeromonas Medium)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aeromonas Medium
(Ryan’s Aeromonas Medium)
Composition per liter:
Agar............................................................................................12.5g
Na 2 S 2 O 3 ....................................................................................10.67g
Proteose peptone...........................................................................5.0g
NaCl..............................................................................................5.0g
Xylose.........................................................................................3.75g
L- Lysine·HCl.................................................................................3.5g
Yeast extract..................................................................................3.0g
Sorbitol .........................................................................................3.0g
Bile salts No. 3..............................................................................3.0g
Inositol..........................................................................................2.5g
L- Arginine·HCl..............................................................................2.0g
Lactose..........................................................................................1.5g
Ferric ammonium citrate...............................................................0.8g
Bromthymol Blue.......................................................................0.04g
Thymol Blue...............................................................................0.04g
pH 8.0 ± 0.1 at 25°C
Source: This medium is available as a dehydrated powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 50°C and aseptically add 5.0mg
of ampicillin. Pour into sterile Petri dishes.
Use: For the isolation and selective differentiation of Aeromonas
hydrophila and other Aeromonas species from clinical and nonclinical
specimens. Aeromonas species appear as small (0.5–1.5mm), dark
green colonies with darker centers.