AFPA (Aspergillus flavus/parasiticus Agar)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AFPA

(Aspergillus flavus/parasiticus Agar)
Composition per liter:
Yeast extract................................................................................20.0g
Agar ............................................................................................15.0g
Peptone........................................................................................10.0g
Ferric ammonium citrate...............................................................0.5g
DChloramphenicol.................................................................100.0mg
ichloran (Botran ® ) .....................................................................2.0mg
pH 6.3 ± 0.2 at 25°C
Source: This medium is available as a dehydrated powder from Oxoid
Unipath.
Preparation of Medium: Add components, except chlroampheni-
col, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat while stirring and bring to boiling. Add 100.0mg
of chloramphenicol. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes.
Use: For the selective isolation and enumeration of Aspergillus flavus
and Aspergillus parasiticus. Colonies of these fungi appear with dark
yellow-orange color on the reverse side.