Methanol Salts Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar, noble..................................................................................20.0g
K 2 HPO 4 .........................................................................................1.2g
KH 2 PO 4 .......................................................................................0.62g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.1g
CaCl 2 ·6H 2 O.................................................................................0.05g
ZnSO 4 ·7H 2 O.............................................................................70.0μg
H 3 BO 3 .......................................................................................10.0μg
MnSO 4 ·5H 2 O............................................................................10.0μg
Na 2 MoO 4 ·2H 2 O ........................................................................10.0μg
CoCl 2 ·6H 2 O ................................................................................5.0μg
CuSO 4 ·5H 2 O...............................................................................5.0μg
FeCl 3 ·6H 2 O................................................................................1.0mg
Methanol....................................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 999.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 1.0mL of filter-steril-
ized methanol. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use: For the cultivation of Hyphomicrobium species, Methylobacillus
glycogenes, Methylobacterium extorquens, Methylobacterium fuji-
sawaense, Methylobacterium mesophilicum, Methylobacterium organo-
philum, Methylobacterium radiotolerans, Methylobacterium rhode-
sianum, Methylobacterium rhodinum, Methylobacterium species,
Methylobacterium zatmanii, Methylomonas species, Methylophilus
methylotrophus, Methylovorus glucosotrophus, Paracoccus species,
Protaminobacter thiaminophaga, and Pseudomonas insueta.