Methanol Urea Mineral Salts Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Urea Mineral Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................2.13g
KH 2 PO 4 .......................................................................................1.36g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.01g
FeSO 4 ·7H 2 O...............................................................................5.0mg
MnSO 4 ·5H 2 O.............................................................................2.5mg
NaMoO 4 ·2H 2 O...........................................................................2.5mg
Urea solution............................................................................30.0mL
Methanol....................................................................................5.0mL
Urea Solution:
Composition per 50.0mL:
Urea.............................................................................................10.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa-
ter and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.
Preparation of Methanol: Filter sterilize 5.0mL of methanol using
a teflon filter.
Preparation of Medium: Add components, except urea solution
and methanol, to distilled/deionized water and bring volume to
965.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 30.0mL of sterile urea solution and 5.0mL of
sterile methanol. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Hyphomicrobium vulgare.