Methanol Mineral Salts Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Mineral Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
NH 4 Cl ...........................................................................................2.0g
(NH 4 ) 2 HPO 4 ..................................................................................2.0g
Yeast extract..................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Fe 2 SO 4 ·7H 2 O..............................................................................0.01g
CaCl 2 ·2H 2 O ................................................................................0.01g
Methanol..................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Filter sterilize methanol. Aseptically add the
sterile methanol to the cooled, sterile basal medium. Mix thoroughly.
Aseptically distribute into sterile Petri dishes or sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas viscogena.