Methanol Medium with 1% Peptone

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Medium with 1% Peptone

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .......................................................................................10.0g
K 2 HPO 4 .........................................................................................7.0g
(NH 4 ) 2 SO 4 ....................................................................................3.0g
KH 2 PO 4 .........................................................................................2.0g
MgSO 4 · 7H 2 O................................................................................0.5g
Yeast extract..................................................................................0.2g
FeSO 4 · 7H 2 O................................................................................0.01g
MnSO 4 ·H 2 O...............................................................................8.0mg
Biotin..........................................................................................0.2μg
Thiamine·HCl.............................................................................0.2μg
Methanol..................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Filter
sterilize methanol. Aseptically add the sterile methanol to the cooled, ster-
ile basal medium. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation and maintenance of Methylobacterium spe-
cies.