Methanol Medium for Achromobacter

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Medium for Achromobacter

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NH 4 Cl ...........................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
NaCl..............................................................................................0.5g
MgSO 4 ..........................................................................................0.2g
Yeast extract..................................................................................0.2g
FeSO 4 .........................................................................................2.0mg
MnCl 2 .........................................................................................2.0mg
Methanol..................................................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 980.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Filter sterilize methanol. Aseptically add the sterile methanol to the
cooled, sterile basal medium. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Achromobacter metha-
nolophila, Methylobacterium rhodesianum, Pseudomonas insueta, and
Pseudomonas polysaccharogenes.