Methanol Medium (ATCC Medium 1096)

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Medium
(ATCC Medium 1096)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NH 4 NO 3 ......................................................................................0.75g
FeCl 3 .........................................................................................0.743g
Methanol.....................................................................................0.45g
MgSO 4 ........................................................................................0.09g
KH 2 PO 4 .....................................................................................0.044g
Na 2 MoO 4 ·2H 2 O .........................................................................0.1mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium under 97%
N 2 + 3% H 2 . Add components, except methanol, to distilled/deionized
water and bring volume to 999.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Filter sterilize methanol. Aseptically add the sterile meth-
anol to the cooled, sterile basal medium. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Ancylobacter species,
Methylobacterium species, and Methanomonas methylovora.