Medium D for Thermus, Modified

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium D for Thermus, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................25.0g
Pancreatic digest of casein............................................................1.0g
Yeast extract..................................................................................1.0g
Salt solution...........................................................................100.0mL
pH 8.2 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaNO 3 ........................................................................................6.89g
Na 2 HPO 4 ·12H 2 O...........................................................................2.8g
KNO 3 ..........................................................................................1.03g
Nitrilotriacetic acid.......................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
CaSO 4 ·2H 2 O.................................................................................0.6g
NaCl............................................................................................0.08g
FeCl 3 ·6H 2 O solution................................................................10.0mL
Trace elements solution ...........................................................10.0mL
FeCl 3 ·6H 2 O Solution:
Composition per 100.0mL:
FeCl 3 ·6H 2 O..............................................................................47.0mg
Preparation of FeCl 3 ·6H 2 O Solution: Add FeCl 3 ·6H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
MnSO 4 ·4H 2 O................................................................................1.7g
ZnSO 4 ·7H 2 O.................................................................................0.5g
H 3 BO 3 ...........................................................................................0.5g
CoCl 2 ·6H 2 O .............................................................................46.0mg
CuSO 4 ·5H 2 O............................................................................25.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................25.0mg
H 2 SO 4 .........................................................................................0.5mL
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Salt Solution: Add nitrilotriacetic acid to 500.0mL
of distilled/deionized water. Dissolve by adjusting pH to 6.5 with
KOH. Add remaining components. Readjust pH to 8.2 with H 2 SO 4 or
KOH. Add distilled/deionized water to 1.0L.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 8.2. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation of Thermus aquaticus.