Medium D for Thermus

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium D for Thermus

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................1.0g
Yeast extract..................................................................................1.0g
NaNO 3 ..........................................................................................0.7g
KNO 3 ............................................................................................0.1g
MgSO 4 ·7H 2 O................................................................................0.1g
Na 2 HPO 4 .......................................................................................0.1g
Nitrilotriacetic acid.......................................................................0.1g
CaSO 4 ·2H 2 O...............................................................................0.06g
NaCl...........................................................................................8.0mg
MnSO 4 ·H 2 O...............................................................................2.2mg
ZnSO 4 ·7H 2 O..............................................................................0.5mg
H 3 BO 3 ........................................................................................0.5mg
FeCl 3 ........................................................................................0.28mg
Na 2 MoO 4 ·2H 2 O.......................................................................0.03mg
CuSO 4 ......................................................................................0.02mg
pH 8.2 ± 0.2 at 25°C
Preparation of Medium: Add nitrilotriacetic acid to 500.0mL of
distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH.
Add remaining components. Readjust pH to 8.2 with H 2 SO 4 or KOH.
Add distilled/deionized water to 1.0L. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Thermus species.