Medium D2

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium D2

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
LiCl...............................................................................................5.0g
Pancreatic digest of casein............................................................4.0g
Yeast extract..................................................................................2.0g
Tris(hydroxymethyl)amino-methane·HCl buffer..........................1.2g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.3g
Polymyxin sulfate solution ......................................................10.0mL
NaN 3 solution...........................................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Polymyxin Sulfate Solution:
Composition per 10.0mL:
Polymyxin sulfate.......................................................................0.04g
Preparation of Polymyxin Sulfate Solution: Add polymyxin
sulfate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize. Use freshly prepared solution.
NaN 3 Solution:
Composition per 10.0mL:
NaN 3 ..........................................................................................2.0mg
Preparation of NaN 3 Solution: Add NaN 3 to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Use freshly prepared solution.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except polymyxin sul-
fate solution and NaN 3 solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile polymyxin sulfate solution and NaN 3 solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation and cultivation of Corynebacterium
species.