Marine Oxidation Fermentation HiVeg Medium (MOF HiVeg Medium)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Oxidation Fermentation HiVeg Medium
(MOF HiVeg Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................9.7g
MnCl 2 ............................................................................................4.4g
Agar ..............................................................................................3.0g
Na 2 SO 4 ..........................................................................................1.6g
Plant hydrolysate...........................................................................1.0g
CaCl 2 .............................................................................................0.9g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
Tris hydroxymethyl aminomethane..............................................0.5g
KCl............................................................................................0.275g
Yeast extract..................................................................................0.1g
NaHCO 3 ......................................................................................0.08g
KBr..............................................................................................0.04g
SrCl 2 ..........................................................................................0.017g
H 3 BO 3 .......................................................................................0.011g
Phenol Red..................................................................................0.01g
Na 2 HPO 4 ....................................................................................4.0mg
Sodium silicate...........................................................................2.0mg
NaFl ...........................................................................................1.2mg
NH 4 NO 3 .....................................................................................0.8mg
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use: For the differentiation of marine bacteria on the basis of fermen-
tative and oxidative metabolism of carbohydrates.