Marine Methylotroph Broth

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Methylotroph Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1003.0mL:

Bis (2-hydroxyethyl) aminotris (hydroxy-
methyl) methane.....................................................................2.0g
KH 2 PO 4 .......................................................................................0.14g
Ferric ammonium citrate.............................................................0.06g
Methanol....................................................................................2.0mL
Vitamin B 12 solution..................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Vitamin  B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add vitamin B 12 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize. Store at 5°C.
Preparation of Medium: Add components, except methanol and
vitamin B 12 solution, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 2.0mL of filter-steril-
ized methanol and 1.0mL of sterile vitamin B 12 solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Alteromonas species, Methylophaga
marina, Methylophaga thalassica, and Methylophilus species.