Marine Peptone Succinate Salts Medium (PSS Medium)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Peptone Succinate Salts Medium
(PSS Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................10.0g
Succinic acid.................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
FeCl 3 ·6H 2 O................................................................................2.0mg
MnSO 4 ·H 2 O...............................................................................2.0mg
Synthetic seawater ........................................................................1.0L
pH 6.8 ± 0.2 at 25°C
Synthetic Seawater:
Composition per liter:
NaCl............................................................................................27.5g
MgCl 2 ............................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................2.0g
KCl................................................................................................1.0g
CaCl 2 .............................................................................................0.5g
FeSO 4 .........................................................................................1.0mg
Preparation of Synthetic Seawater: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to 1.0L of synthetic sea-
water. Mix thoroughly. Gently heat while stirring and bring to boiling.
Adjust pH to 6.8 with KOH. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Oceanospirillum beijer-
inckii and Oceanospirillum multiglobuliferum.