Acidophilium Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidophilium Agar
Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
pH 3.5 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
Agar ............................................................................................12.0g
Preparation of Solution A:  Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
Solution B:
Composition per 500.0mL:
Mannitol........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
(NH 4 ) 2 SO 4 .....................................................................................0.1g
Tryptone soya broth......................................................................0.1g
KCl...........................................................................................50.0mg
KH 2 PO 4 ....................................................................................50.0mg
Ca(NO 3 ) 2 ..................................................................................10.0mg
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Bring pH to 3.5. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Aseptically combine 500.0mL of solu-
tion A with 500.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or aseptically distribute into sterile tubes.
Use: For the cultivation and maintenance of Acidiphilium cryptum and
other Acidiphilium species.