Aciduliprofundum Medium (DSMZ Medium 1083)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aciduliprofundum Medium
(DSMZ Medium 1083)
Composition per liter:
NaCl ...........................................................................................30.0g
MgSO 4 ·7H 2 O ....................................................................... 3.5g
MgCl 2 ·6H 2 O ...................................................................... 2.75g
CaCl 2 ·2H 2 O ....................................................................... 0.38g
KCl .............................................................................................0.33g
NaBr ...........................................................................................0.05g
(NH 4 ) 2 SO 4 ......................................................................... 0.10g
KH 2 PO 4 ............................................................................ 0.28g
Wolfe's mineral elixir.................................................................1.0mL
Resazurin ..................................................................................0.5mg
Sodium citrate ............................................................................2.94g
Yeast extract .................................................................................1.0g
Tryptone .......................................................................................1.0g
Sulfur, powdered ........................................................................10.0g
Na 2 S·9H 2 O solution .........................................................10.0mL
pH 4.5 ± 0.2 at 25°C
Wolfe’s Mineral Elixir:
Composition per liter :
MgSO 4 ·7H 2 O..............................................................................30.0g
NaCl............................................................................................10.0g
MnSO 4 ·2H 2 O................................................................................5.0g
(NH 4 ) 2 NiSO 4 ·6H 2 O ......................................................................2.8g
CoCl 2 ·6H 2 O ..................................................................................1.8g
ZnSO 4 ·7H 2 O.................................................................................1.8g
FeSO 4 ·7H 2 O..................................................................................1.0g
CaCl 2 ·2H 2 O...................................................................................1.0g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.18g
CuSO 4 ·5H 2 O.................................................................................0.1g
H 3 BO 3 ...........................................................................................0.1g
Na 2 MoO 4 ·2H 2 O............................................................................0.1g
Na 2 SeO 4 ........................................................................................0.1g
Na 2 WO 4 ·2H 2 O..............................................................................0.1g
Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of dis-
tilled/deionized water to 1.0 with dilute H 2 SO 4 . Add remaining com-
ponents one at a time. Mix throughly to dissolve.
Na 2 S·9H 2 O Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Adjust pH to 4.5.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution and sulfur, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1
min. Cool to room temperature while sparging with 80% N 2 + 20%
CO 2 . Distribute into serum bottles containing the sulfur. Distribute un-
der 80% N 2 + 20% CO 2 , e.g., 20mL into 120mL serum bottles. Auto-
clave for 60 min at 3 psi pressure–105°C. Cool to 25°C. Aseptically
inject Na 2 S·9H 2 O solution, 0.2mL per 20mL medium. Mix thoroughly.
Adjust pH to 4.5.
Use: For the cultivation of Aciduliprofundum sp.