Acidophilic Bacillus stearothermophilus Broth

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidophilic Bacillus
stearothermophilus Broth
Composition per liter:
Soluble starch..............................................................................10.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................0.5g
MnCl 2 ·4H 2 O .................................................................................0.5g
pH 5.0 ± 0.2 at 25°C
Preparation of Medium: Dissolve all components in 1.0L of dis-
tilled/deionized water. Mix thoroughly. Gently heat and bring to boiling.
Adjust to pH 5.0. Autoclave for 15 min at 15 psi pressure–121°C. Precip-
itate will dissolve after cooling and mixing.
Use: For the cultivation and maintenance of Bacillus stearothermo-
philus and other acidophilic Bacillus species.