Acidophilic Bacillus stearothermophilus Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidophilic Bacillus stearothermophilus Agar
Composition per liter:
Part B.....................................................................................600.0mL
Part A.....................................................................................400.0mL
pH 5.0 ± 0.2 at 25°C
Part A:
Composition per 400.0mL:
Soluble starch..............................................................................10.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................0.5g
MnCl 2 ·4H 2 O .................................................................................0.5g
Preparation of Part A:  Add components to distilled/deionized wa-
ter and bring volume to 400.0mL. Mix thoroughly. Gently heat and
bring to boiling. Adjust pH to 4.7. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°C.
Part B:
Composition per 600.0mL:
Agar............................................................................................20.0g
Preparation of Part B: Add agar to distilled/deionized water and
bring volume to 600.0mL. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C.
Preparation of Medium: Aseptically combine solution A and solu-
tion B. Mix thoroughly. Adjust pH to 5.0. Pour into sterile Petri dishes.
Use: For the cultivation and maintenance of Bacillus stearothermo-
philus and other acidophilic Bacillus species.