Acidomonas methanolica Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidomonas methanolica Agar
Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
pH 4.0 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
Glucose .......................................................................................20.0g
Yeast extract..................................................................................5.0g
(NH 4 ) 2 SO 4 ....................................................................................3.0g
KH 2 PO 4 ........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.7g
NaCl..............................................................................................0.5g
Ca(NO 3 ) 2 ·4H 2 O............................................................................0.4g
K 2 HPO 4 ·3H 2 O............................................................................0.16g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar............................................................................................20.0g
Preparation of Solution B:  Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically mix 500.0mL of solution A
and 500.0mL of solution B. Mix thoroughly. Aseptically adjust pH to
4.0. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Acidomonas methanolica