Marine Agar (DSMZ Medium 123)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Agar
(DSMZ Medium 123)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Tryptone......................................................................................10.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................1.0g
Synthetic seawater ........................................................................1.0L
pH 7.8 ± 0.2 at 25°C
Synthetic Seawater:
Composition per liter:
NaCl............................................................................................24.0g
MgCl 2 ·6H 2 O................................................................................11.0g
Na 2 SO 4 ..........................................................................................4.0g
CaCl 2 ·6H 2 O ..................................................................................2.0g
KCl................................................................................................0.7g
KBr ...............................................................................................0.1g
SrCl 2 ·6H 2 O .................................................................................0.04g
H 3 BO 3 .........................................................................................0.03g
NaSiO 3 ·9H 2 O.............................................................................5.0mg
NaF ............................................................................................3.0mg
NH 4 NO 3 .....................................................................................2.0mg
Fe 3 PO 4 ·4H 2 O.............................................................................1.0mg
Preparation of Synthetic Seawater: Add components to distilled
water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add agar, tryptone, peptone, and yeast
extract to synthetic seawater and bring volume to 1.0L. Mix thorough-
ly. Adjust pH to 7.8. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Halobacillus halophilus,
Halomonas spp., Vibrio harveyi, Cobetia marina, and Ruegeria atlan-
tica.