Marine Agar 2216 (DSMZ Medium 604)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Agar 2216
(DSMZ Medium 604)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................19.45g
Agar............................................................................................15.0g
MgCl 2 ............................................................................................8.8g
Peptone .........................................................................................5.0g
Na 2 SO 3 ........................................................................................3.24g
CaCl 2 .............................................................................................1.8g
Yeast extract..................................................................................1.0g
KCl..............................................................................................0.55g
NaHCO 3 ......................................................................................0.16g
Ferric citrate..................................................................................0.1g
KBr .............................................................................................0.08g
SrCl 2 ............................................................................................0.03g
H 3 BO 3 .........................................................................................0.02g
Na 2 HPO 4 ....................................................................................8.0mg
Na 2 SiO 3 ......................................................................................4.0mg
NaF ............................................................................................2.4mg
NH 4 NO 3 .....................................................................................1.6mg
pH 7.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation and maintenance of Hyphomonas spp., Ocean-
ospirillum spp., Hyphomicrobium indicum, Psychroflexus gondwanen-
sis=Flavobacterium gondwanense, Salegentibacter salegens=Fla-
vobacterium  salegens,  Psychromonas  antarctica,  Sulfitobacter
mediterraneus, Thalassomonas viridans, Vibrio spp., Marinospirillum
minutulum=Oceanospirillum minutulum, Terasakiella pusilla=Oceano-
spirillum pusillum, Pseudoalteromonas atlantica=Alteromonas atlan-
tica, Pseudomonas atlantica, Roseobacter spp., Erythrobacter longus,
Pseudospirillum japonicum=Oceanospirillum japonicum, Marino-

bacter hydrocarbonoclasticus (Pseudomonas nautica), Psychrobacter

spp., and Moritella japonica. For the isolation, cultivation, and mainte-
nance of a wide variety of heterotrophic marine bacteria.