Maricaulis Medium (DSMZ Medium 1025)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Maricaulis Medium
(DSMZ Medium 1025)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sea salts, Sigma ..........................................................................30.0g
NH 4 Cl ...........................................................................................0.5g
Peptone yeast extract solution..................................................20.0mL
Glucose solution ........................................................................2.0mL
Riboflavin solution ....................................................................5.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution :
Composition per 10.0mL:
Glucose .........................................................................................5.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Riboflavin Solution :
Composition per 10.0mL:
Riboflavin ..................................................................................2.0mg
Preparation of Riboflavin Solution:  Add riboflavin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Peptone Yeast Extract Solution :
Composition per 100.0mL:
Peptone........................................................................................10.0g
Yeast extract..................................................................................5.0g
Preparation of Peptone Yeast Extract Solution:  Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except riboflavin, glu-
cose, and peptone yeast extract solutions, to distilled/deionized water
and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.2. Gen-
tly heat while stirring and bring to boiling. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to room temperature. Asep-
tically add riboflavin, glucose, and peptone yeast extract solutions.
Use: For the cultivation of Maricaulis spp.