Acidomonas Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidomonas Agar
Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
Solution A:
Composition per 500.0mL:
Glucose .......................................................................................10.0g
Peptone..........................................................................................5.0g
Malt extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.0. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar ............................................................................................20.0g
Preparation of Solution B:  Add 20.0g of agar to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C.
Preparation of Medium: Aseptically mix 500.0mL of solution A
with 500.0mL of solution B. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation and maintenance of Acidomonas methanolica.