Acidimicrobium Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidimicrobium Medium
Composition per liter:
MgSO 4 ·7H 2 O................................................................................0.5g
(NH 4 ) 2 SO 4 ....................................................................................0.4g
K 2 HPO 4 .........................................................................................0.2g
KCl................................................................................................0.1g
FeSO 4 ·7H 2 O.............................................................................10.0mg
Yeast extract solution...............................................................20.0mL
pH 2.0 ± 0.2 at 25°C
Yeast Extract Solution:
Composition per 20.0mL:
Yeast extract................................................................................10.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except yeast extract so-
lution, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Adjust pH to 2.0 with H 2 SO 4 . Autoclave for 15 min at 15
psi pressure–121°C. Aseptically add 20.0mL of sterile yeast extract so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the heterotrophic cultivation of Sulfobacillus acidophilus.