Acidified Potato Dextrose Agar (APDA)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidified Potato Dextrose Agar
(APDA)
Composition per liter:
Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Potatoes, infusion from..........................................................500.0mL
Lactic acid solution....................................................................5.0mL
pH 5.6 ± 0.2 at 25°C
Potato Infusion:
Composition per 500.0mL:
Potatoes.....................................................................................300.0g
Preparation of Potato Infusion:  Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate.
Lactic Acid Solution:
Composition per 10.0mL:
Lactic acid.....................................................................................2.5g
Preparation of Lactic Acid Solution: Add lactic acid to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except lactic acid solu-
tion, s to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 20 min at 15 psi pressure–121°C. Cool to 50°C. Add
5.0mL of lactic acid solution. Mix thoroughly. Pour into sterile Petri
dishes or leave in tubes.
Use: For the isolation, cultivation, and identification of oak wilt fungi.