Acidic Tomato Medium for Leuconostoc

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidic Tomato Medium for Leuconostoc
Composition per liter:
Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
Peptone........................................................................................10.0g
Yeast extract..................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
Tomato juice...........................................................................250.0mL
pH 4.8 ± 0.2 at 25°C
Preparation of Medium: Add solid components to 750.0mL of dis-
tilled/deionized water. Add tomato juice. Mix well and warm gently
until dissolved. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes.
Use: For the cultivation and maintenance of Leuconostoc oenos and
other Leuconostoc species.