Acidic Rhodospirillaceae Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidic Rhodospirillaceae Medium
Composition per 1006.0mL:
Disodium succinate.......................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
Yeast extract..................................................................................0.2g
CaCl 2 ·H 2 O................................................................................50.0mg
Ferric citrate solution.................................................................5.0mL
Trace elements solution .............................................................1.0mL
Ferric Citrate Solution:
Composition per 100.0mL:
Ferric citrate..................................................................................0.1g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except ferric citrate so-
lution and trace elements solution, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 5.0mL of sterile ferric citrate solution
and 1.0mL of sterile trace elements solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation of Rhodopseudomonas acidophila.