Acidicaldus Medium (DSMZ Medium 1038)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidicaldus Medium
(DSMZ Medium 1038)
Composition per liter:
MgSO 4 ·7H 2 O................................................................................0.5g
(NH 4 ) 2 SO 4 ..................................................................................0.45g
KCl .............................................................................................0.05g
KH 2 PO 4 ...................................................................................... 0.05g
Ca(NO 3 ) 2 ·4H 2 O .......................................................................14.0mg
Glucose solution ......................................................................10.0mL
Yeast extract solution...............................................................10.0mL
pH 2.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose ........................................................................................1.0g
Preparation of Glucose Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract................................................................................. 0.2g
Preparation of Yeast Extract Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-
ature.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to room temperature. Adjust pH to 2.5.
Aseptically add 10.0mL sterile glucose solution and 10.0mL sterile
yeast extract solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes or flasks.
Use: For the cultivation and maintenance of Acidicaldus organivorans.