M63 Medium, 5X

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M63 Medium, 5X

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 ......................................................................................68.0g
(NH 4 ) 2 SO 4 ..................................................................................10.0g
FeSO 4 ·7H 2 O..............................................................................2.5mg
Carbohydrate solution..............................................................10.0mL
MgSO 4 ·7H 2 O solution...............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................20.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Glucose or
glycerol may be used. Mix thoroughly. Filter sterilize.
MgSO 4 ·7H 2 O Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O............................................................................24.65g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate so-
lution and MgSO 4 ·7H 2 O solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. To prepare
medium for use (1×), aseptically dilute 200.0mL of 5× stock solution
with 789.0mL of sterile distilled/deionized water. Aseptically add
10.0mL of sterile carbohydrate solution and 1.0mL of sterile
MgSO 4 ·7H 2 O solution. Mix thoroughly. Aseptically distribute into ster-

ile tubes or flasks.

Use: For the cultivation of Escherichia coli.