MA Medium

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

MA Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

Peptone........................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Ribonucleic acid from Torula yeast..............................................1.0g
Asolectin.......................................................................................0.2g
Artificial seawater..................................................................500.0mL
Vitamin solution.........................................................................2.0mL
Preparation of Medium: Emulsify asolectin in warm, distilled wa-
ter before adding remaining powdered ingredients. Adjust pH to 7.2.
Add vitamin mix and artificial seawater; readjust to pH 7.2, if neces-
sary. Dispense 5.0mL per 16 x 125mm screw-capped test tube and au-
toclave at 121°C for 15 min.
Artificial Seawater:
Composition per 500.0mL:
Aqua-Marin sea salts ..................................................................20.8g
Source:  Aqua-Marin sea salts are available from Aquatrol, Inc., Ana-
heim, CA.
Preparation of Artificial Seawater: Add Aqua-Marin sea salts to
distilled/deionized water and bring volume to 500.0mL. Mix thorough-
ly.
Vitamin Solution:
Composition per 110.0mL:
Thiamine·HCl )......................................................................150.0mg
Calcium  D -(+)-pantothenate...................................................100.0mg
Folic acid..................................................................................50.0mg
Nicotinamide............................................................................50.0mg
Pyridoxal·HCl..........................................................................50.0mg
Riboflavin ................................................................................50.0mg
DL -6-Thioctic acid......................................................................1.0mg
Biotin solution..........................................................................10.0mL
Biotin Solution:
Composition per 10.0mL:
Biotin .......................................................................................0.01mg
Preparation of Biotin Solution: Add biotin to 10.0mL of absolute
ethanol. Mix thoroughly.
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. For
long-term storage, preserve under nitrogen at −20°C.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
add 2.0mL of sterile vitamin solution. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use: For the cultivation of Anophryoides soldoi, Metanophrys
diminuta, Mesanophrys chesapeakensis, Miamiensis avidus, Parau-
ronema acutum, and Paranophrys species.