M17 Medium for Lactic Streptococci (DSMZ Medium 449)

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Medium for Lactic Streptococci
(DSMZ Medium 449)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 -ß-glycerophosphate.............................................................19.0g
Peptone from casein......................................................................5.0g
Soy peptone ..................................................................................5.0g
Peptone bacteriological.................................................................5.0g
Yeast extract..................................................................................2.5g
Ascorbic acid................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.25g
Lactose solution.......................................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Lactose Solution:
Composition per 10.0mL:
Lactose..........................................................................................5.0g
Preparation of Lactose Solution: Add lactose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except lactose solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 10.0mL sterile lactose solution. Aseptically distribute
to sterile tubes or flasks.
Use: For the cultivation and maintenance of Lactococcus lactis subsp.
lactis=Streptococcus lactis.