M17 Medium, Modified

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.2mL:

Disodium-ß-glycerophosphate......................................................9.5g
Pancreatic digest of casein............................................................5.0g
Meat peptone ................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Yeast extract..................................................................................2.5g
Ascorbic acid................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.25g
Lactose solution.......................................................................50.0mL
CaCl 2 solution............................................................................1.2mL
pH 7.15 ± 0.05 at 25°C
Lactose Solution:
Composition per 50.0mL:
Lactose..........................................................................................8.0g
Preparation of Lactose Solution:  Add lactose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O.................................................................................14.7g
Preparation of CaCl 2 Solution:  Add CaCl 2 ·2H 2 O to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except lactose solution
and CaCl 2 solution, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 50.0mL of sterile lactose solution and 1.2mL
of sterile CaCl 2 solution. Mix thoroughly. Aseptically adjust pH to 7.15
± 0.05. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Streptococcus thermophilus.