M17 Medium for Filomicrobium fusiforme (DSMZ Medium 768)

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Medium for Filomicrobium fusiforme
(DSMZ Medium 768)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na-acetate .....................................................................................1.0g
KNO 3 ............................................................................................1.0g
Artificial seawater, concentrated............................................500.0mL
Hutner's salts solution..............................................................20.0mL
Vitamin solution.......................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Hutner’s Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O...............................................................................3.335g
FeSO 4 ·7H 2 O.............................................................................99.0mg
(NH 4 ) 6 MoO 7 O 24 ·4H 2 O ............................................................9.25mg
“Metals 44”..............................................................................50.0mL
“Metals 44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.............................................................................1.095g
FeSO 4 ·7H 2 O..................................................................................0.5g
Sodium EDTA.............................................................................0.25g
MnSO 4 ·H2O..............................................................................0.154g
CuSO 4 ·5H 2 O............................................................................39.2mg
Co(NO 3 ) 2 ·6H 2 O .......................................................................24.8mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................17.7mg
Preparation of “Metals 44”: Add sodium EDTA to distilled/de-
ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few
drops of concentrated H 2 SO 4 to retard precipitation of heavy metal
ions. Add remaining components. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water.
Preparation of Hutner’s Salts Solution:  Add nitrilotriacetic acid
to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH.
Add remaining components. Add distilled/deionized water to 1.0L.
Adjust pH to 6.8.
Artificial Seawater, Concentrated:
Composition per liter:
NaCl..........................................................................................70.43g
MgCl 2 ·6H 2 O..............................................................................31.86g
Na 2 SO 4 ......................................................................................11.75g
CaCl 2 ·2H 2 O.................................................................................4.35g
NaHCO 3 ......................................................................................2.88g
KCl..............................................................................................1.99g
KBr..............................................................................................0.29g
H 3 BO 3 .........................................................................................0.08g
Preparation of Artificial Seawater:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Vitamin Solution:
Composition per liter:
Riboflavin ..................................................................................5.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Ca-pantothenate.........................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except artificial sea wa-
ter and vitamin solution, to distilled/deionized water and bring volume
to 490.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at
15 psi pressure–121°C. Cool to room temperature. Aseptically add
500.0mL artificial sea water and 10.0mL vitamin solution. Mix thor-
oughly. Aseptically and anaerobically distribute into sterile tubes or bot-
tles.
Use:  For the cultivation of Filomicrobium fusiforme.