M17 Agar

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Disodium β-glycerophosphate....................................................19.0g
Agar ............................................................................................11.0g
Beef extract...................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Yeast extract..................................................................................2.5g
Ascorbic acid ................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.25g
Lactose solution.......................................................................50.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Lactose Solution:
Composition per 100.0mL:
Lactose........................................................................................10.0g
Preparation of Lactose Solution: Add lactose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except lactose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile
lactose solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation and maintenance of streptococci and their bac-
teriophages. Also used for the cultivation and maintenance of starter
cultures for cheese and yogurt manufacture as well as detecting strep-
tococcal mutants which are unable to ferment lactose.