M17 Agar (LMG Medium 261)

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Agar
(LMG Medium 261)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Disodium β-glycerophosphate....................................................19.0g
Agar ............................................................................................11.0g
Polypeptone™...............................................................................5.0g
Beef extract...................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Yeast extract..................................................................................2.5g
Ascorbic acid ................................................................................0.5g
Lactose solution.......................................................................50.0mL
MgSO 4 ·7H 2 O (1M solution)......................................................1.0mL
pH 6.9 ± 0.2 at 25°C
Lactose Solution:
Composition per 100.0mL:
Lactose........................................................................................10.0g
Preparation of Lactose Solution: Add lactose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except lactose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile
lactose solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation of Streptococcus thermophilus and for the cul-
tivation and maintenance of streptococci and their bacteriophages.
Also used for the cultivation and maintenance of starter cultures for
cheese and yogurt manufacture as well as detecting streptococcal
mutants that are unable to ferment lactose