M17 Broth

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Disodium β-glycerophosphate....................................................19.0g
Beef extract...................................................................................5.0g
Lactose..........................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Pancreatic digest of casein............................................................2.5g
Peptic digest of animal tissue .......................................................2.5g
Yeast extract..................................................................................2.5g
Ascorbic acid................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.25g
pH 7.15 ± 0.05 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of streptococci and their bac-
teriophages. Also used for the cultivation and maintenance of starter cul-
tures for cheese and yogurt manufacture as well as detecting streptococ-
cal mutants that are unable to ferment lactose.