M16 Agar

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M16 Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Beef extract...................................................................................5.0g
Pancreatic digest of soybean meal................................................5.0g
Polypeptone ™ ...............................................................................5.0g
Sodium acetate·3H 2 O....................................................................3.0g
Yeast extract..................................................................................2.5g
Ascorbic acid................................................................................0.5g
Carbohydrate solution..............................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 50.0mL:
Lactose or glucose........................................................................5.0g
Preparation of Carbohydrate Solution: Add lactose or glucose
to distilled/deionized water and bring volume to 50.0mL. Mix thor-

oughly. Filter sterilize.

Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 950.0mL.
Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2 with
2N NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 50.0mL of sterile carbohydrate solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of lactobacilli from cheddar cheese.