Lysozyme Broth

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysozyme Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1005.0mL:

Basal glycerol broth......................................................................1.0L
Lysozyme solution.....................................................................5.0mL
Basal Glycerol Broth:
Composition per liter:
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
Glycerol ...................................................................................70.0mL
Preparation of Basal Glycerol Broth: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis-
tribute 500.0mL of the broth into screw-capped tubes in 5.0mL
volumes. Autoclave the tubes and the flask with the remaining broth
for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Lysozyme Solution:
Composition per 100.0mL:
Lysozyme......................................................................................0.1g
HCl (0.01N solution)..............................................................100.0mL
Preparation of Lysozyme Solution: Add lysozyme to 100.0mL
of HCl solution. Mix thoroughly. Filter sterilize. Store for up to 1 week
at 4°C.
Preparation of Medium: Add 5.0mL of the sterile lysozyme solu-
tion to 95.0mL of the cooled, sterile basal glycerol broth. Mix thor-
oughly. Aseptically distribute into sterile screw-capped tubes in 5.0mL
volumes.
Use: For the cultivation and differentiation of Nocardia asteroides, Strep-
tomyces griseus, and Actinomadura madurae based on sensitivity to
lysozyme. Nocardia asteroides grows well in both the basal glycerol broth
and the lysozyme broth. Actinomadura madurae and Streptomyces griseus
grow well in the basal glycerol broth but not in the lysozyme broth.