Lysozyme Broth

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysozyme Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Nutrient broth................................................................................1.0L
Lysozyme solution...................................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Nutrient Broth:
Composition per liter:
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Source:  Nutrient broth is available as a premixed powder from BD
Diagnostic Systems.
Preparation of Nutrient Broth: Add components to distilled/de-

ionized water and bring volume to 1.0L. Mix thoroughly. Distribute

into bottles in 99.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C.
Lysozyme Solution:
Composition per 100.0mL:
Lysozyme......................................................................................0.1g
Preparation of Lysozyme Solution: Add lysozyme to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add 1.0mL of sterile lysozyme solution
to 99.0mL of cooled, sterile nutrient broth. Mix thoroughly. Aseptical-
ly distribute into sterile tubes in 2.5mL volumes.
Use: For the cultivation and differentiation of Bacillus cereus in foods.
Bacillus cereus is resistant to lysozyme and will grow in this medium.