Lysobacter deserti Medium (DSMZ Medium 1060)

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

Lysobacter deserti Medium
(DSMZ Medium 1060)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................700.0mL
Solution B..............................................................................300.0mL
pH 8.1 ± 0.2 at 25°C
Solution A :
Composition per 700.0mL:
Agar............................................................................................15.0g
Glucose ......................................................................................10.0g
Peptone .........................................................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 ........................................................................................1.0g
MgSO 4 · 7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................40.0g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 700.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Solution B :
Composition per 300.0mL:
NaCl............................................................................................20.0g
Na 2 CO 3 .........................................................................................1.0g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 300.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Preparation of Medium: Aseptically combine 700.0mL sterile so-
lution A and 300.0mL sterile solution B. Mix thoroughly. Adjust pH to
8.1. Pour into Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Lysobacter deserti.